All reagents for western blot are mostly purchased by labs, but if you still make your own reagents, here are some recipes I used during graduate school. These reagents are compatible with all pre-made gels. If you really want a recipe for SDS protein gels, let me know…
10x Transfer Buffer (1L):
144g Glycine
30g Tris
10x TBS (2L):
48.4g [...]
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western blotting reagent recipes
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