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DT40s are a cell culture line of chicken lymphocyte B cells. They exhibit a high rate of targeted (and random) integration of transfected DNA constructs and are ideal for stable transfection. They can, however, be a royal pain to culture. Anyone who has worked with DT40s can attest to the fact that it is probably [...]
All reagents for western blot are mostly purchased by labs, but if you still make your own reagents, here are some recipes I used during graduate school. These reagents are compatible with all pre-made gels. If you really want a recipe for SDS protein gels, let me know…
10x Transfer Buffer (1L):
144g Glycine
30g Tris
10x TBS (2L):
48.4g [...]
Sodium Borate Electrophoresis Buffer (SBE buffer) is an excellent alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. SBE buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.
Brody and Kern described sodium borate buffer for nucleic acid electrophoresis several years ago (Sodium boric acid: a Tris-free, cooler [...]
