http://www.mudphudder.com/2009/09/flow-cytometry-protocols-facilities-and-online-protocols/ an academic medicine weblog Fri, 18 May 2012 03:26:35 +0000 http://wordpress.org/?v=2.7.1 hourly 1 http://www.mudphudder.com/2009/09/flow-cytometry-protocols-facilities-and-online-protocols/comment-page-1/#comment-10699 mudphudder Thu, 08 Oct 2009 00:03:03 +0000 http://www.mudphudder.com/?p=3014#comment-10699 Hey Dude- sorry for the late reply. I actually don't know about that. I never needed an antibody to the DJ component of the VDJ. I'm sure you've checked the usual suspects in terms of commercial sources (e.g. BD) but when that failed for me, I would always go through papers looking for someone who had the antibody I needed and then asked them for some. But you've probably already done that too, so I guess my input is completely worthless here. Hey Dude- sorry for the late reply. I actually don’t know about that. I never needed an antibody to the DJ component of the VDJ. I’m sure you’ve checked the usual suspects in terms of commercial sources (e.g. BD) but when that failed for me, I would always go through papers looking for someone who had the antibody I needed and then asked them for some. But you’ve probably already done that too, so I guess my input is completely worthless here.

]]> http://www.mudphudder.com/2009/09/flow-cytometry-protocols-facilities-and-online-protocols/comment-page-1/#comment-10522 Andrew Sat, 03 Oct 2009 18:07:45 +0000 http://www.mudphudder.com/?p=3014#comment-10522 What's up Mudphudder? Seeing this flow cytometry list reminded me of a technical problem I'm having regarding flow detection of a specific T cell receptor. We have a transgenic TCR mouse with human alpha/beta variable regions and mouse constant regions. Unfortunately, there are no antibodies to the human alpha/beta types of this clone (Vb5.4, Va13.2). Very frustrating - especially because we've had a tetramer reagent made for this class II restricted TCR, but it's VERY lousy. Do you know if antibodies exist to the DJ component of the VDJ? I obviously can't probe for the constant region since the entire mouse TCR repertoire would be detected. Other than this, I think we're sol.... hope internship is going well. What’s up Mudphudder? Seeing this flow cytometry list reminded me of a technical problem I’m having regarding flow detection of a specific T cell receptor. We have a transgenic TCR mouse with human alpha/beta variable regions and mouse constant regions. Unfortunately, there are no antibodies to the human alpha/beta types of this clone (Vb5.4, Va13.2). Very frustrating - especially because we’ve had a tetramer reagent made for this class II restricted TCR, but it’s VERY lousy. Do you know if antibodies exist to the DJ component of the VDJ? I obviously can’t probe for the constant region since the entire mouse TCR repertoire would be detected. Other than this, I think we’re sol…. hope internship is going well.

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