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scansite: an online resource for detecting motifs of protein kinase phosphorylation

Scansite was developed by Michael Yaffe, a world reknowned cell signaling (in particular, protein phosphorylation) researcher at MIT.  Scansite is an online resource to search for motifs within proteins that are likely to be phosphorylated by specific protein kinases or bind to domains such as SH2 domains, 14-3-3 domains or PDZ domains.

Optimal phosphorylation sites for particular protein Ser/Thr kinases or protein-Tyr kinases are predicted using the matrix of selectivity values for amino acids at each position relative to the phosphorylation site as determined from the oriented peptide library technique described by Songyang et al., 1994, Current Biology 4, 973-982 and Songyang et al., 1995, Nature 373, 536-539.

Optimal binding sites for SH2 domains, PDZ domains, 14-3-3 domains and other domains are determined using the matrix of selectivity values for amino acids at each position relative to an orienting residue as determined by the oriented peptide library technique described in Songyang et al., 1993 Cell 72, 767-778, Songyang et al., 1997 Science 275, 73-77 and Yaffe et al., 1997 Cell 91, 961-971.

The same matrices of selectivity values are used in an approach to provide relative scores of candidate motifs in protein sequences evaluated.  Proteins can be scanned by accession number or with an input sequence.   One or many proteins can be scanned at a time.  Scansite will also allow you to scan the genomes of various species for sequences that are compatible with over 60 protein kinase recognition sites. 

If you are interested in or work on protein phosphorylation, check out Scansite!

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2 Responses to “scansite: an online resource for detecting motifs of protein kinase phosphorylation”

  1. 1
    Comrade PhysioProf:

    These things are for fucking wimps. Back in my day, real men identified binding and phosphorylation motifs with their own eyes! Now get offa my fucking lawn!

  2. 2
    mudphudder:

    Whatever–that you and the other real men were writing out protein sequences with a lump of coal by candle light “in your day” isn’t reason to be hating on modern technology.

    HAHAHAHAHAHAHAHAHAHAHAHAHAHAHA!!!!!!!!!!!!!!!!!

    You know you love this stuff.

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