western blotting reagent recipes

All reagents for western blot are mostly purchased by labs, but if you still make your own reagents, here are some recipes I used during graduate school. These reagents are compatible with all pre-made gels. If you really want a recipe for SDS protein gels, let me know…

10x Transfer Buffer (1L):
144g Glycine
30g Tris

10x TBS (2L):
48.4g Tris Base 7.7
160g NaCl
Fill to 2L with dH20
pH to 7.6

Blocking buffer:
1 part 10x TBS
9 parts dH2O
5% mass/vol non-fat powdered milk
0.1% vol/vol Tween20

Wash Buffer:
1 part 10x TBS
9 parts dH20
0.5% Tween20